Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research

ORCID
0000-0002-8669-3359
Zugehörigkeit
Department of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Postępu 36A, 05-552 Magdalenka, Poland
Skopek, Rafał;
ORCID
0000-0002-0399-5280
Zugehörigkeit
Department of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Postępu 36A, 05-552 Magdalenka, Poland
Palusińska, Małgorzata;
Zugehörigkeit
Department of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Postępu 36A, 05-552 Magdalenka, Poland
Kaczor-Keller, Katarzyna;
ORCID
0000-0001-8650-1161
Zugehörigkeit
Department of Physiological Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, 02-787 Warsaw, Poland
Pingwara, Rafał;
ORCID
0000-0001-9814-2899
Zugehörigkeit
Polbionica Sp. z.o.o., Aleja Prymasa Tysiąclecia 79A, 01-242 Warsaw, Poland
Papierniak-Wyglądała, Anna;
GND
133923118
Zugehörigkeit
Department of Hematology and Medical Oncology, Clinic of Internal Medicine II, Jena University Hospital, 07747 Jena, Germany
Schenk, Tino;
ORCID
0000-0002-0539-0680
Zugehörigkeit
Faculty of Medical Sciences and Health Sciences, Kazimierz Pulaski University of Technology and Humanities, 26-600 Radom, Poland
Lewicki, Sławomir;
Zugehörigkeit
Department of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Postępu 36A, 05-552 Magdalenka, Poland
Zelent, Artur;
ORCID
0000-0003-0148-541X
Zugehörigkeit
Department of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Postępu 36A, 05-552 Magdalenka, Poland
Szymański, Łukasz

Immortalized cell lines are widely used in vitro tools in oncology and hematology research. While these cell lines represent artificial systems and may accumulate genetic aberrations with each passage, they are still considered valuable models for pilot, preliminary, and screening studies. Despite their limitations, cell lines are cost-effective and provide repeatable and comparable results. Choosing the appropriate cell line for acute myeloid leukemia (AML) research is crucial for obtaining reliable and relevant results. Several factors should be considered when selecting a cell line for AML research, such as specific markers and genetic abnormalities associated with different subtypes of AML. It is also essential to evaluate the karyotype and mutational profile of the cell line, as these can influence the behavior and response to the treatment of the cells. In this review, we evaluate immortalized AML cell lines and discuss the issues surrounding them concerning the revised World Health Organization and the French–American–British classifications.

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