Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex

GND
1301877840
ORCID
0000-0003-0345-2507
Zugehörigkeit
Institute of Microbiology, Friedrich Schiller University Jena, Neugasse 25, 07743 Jena, Germany
Harpke, Marie;
GND
1300393459
Zugehörigkeit
Institute of Microbiology, Friedrich Schiller University Jena, Neugasse 25, 07743 Jena, Germany
Pietschmann, Sebastian;
GND
1047788152
ORCID
0000-0002-4192-490X
Zugehörigkeit
Mass Spectrometry Platform, Friedrich Schiller University Jena, Humboldtstr. 8, 07743 Jena, Germany
Ueberschaar, Nico;
ORCID
0000-0001-8984-3853
Zugehörigkeit
Leibniz Institute for Natural Product Research and Infection Biology, Department of Molecular and Applied Microbiology, Adolf-Reichwein-St. 23, 07745 Jena, Germany
Krüger, Thomas;
ORCID
0000-0002-9493-6402
Zugehörigkeit
Leibniz Institute for Natural Product Research and Infection Biology, Department of Molecular and Applied Microbiology, Adolf-Reichwein-St. 23, 07745 Jena, Germany
Kniemeyer, Olaf;
GND
1138445681
Zugehörigkeit
Institute of Microbiology, Friedrich Schiller University Jena, Neugasse 25, 07743 Jena, Germany
Brakhage, Axel A.;
GND
12341461X
Zugehörigkeit
Elektronenmikroskopisches Zentrum, Universitätsklinikum Jena, Ziegelmühlenweg 1, 07743 Jena, Germany
Nietzsche, Sandor;
GND
143604783
ORCID
0000-0002-8777-8004
Zugehörigkeit
Institute of Microbiology, Friedrich Schiller University Jena, Neugasse 25, 07743 Jena, Germany
Kothe, Erika

Three strains of the Aspergillus versicolor complex were isolated from a salty marsh at a former uranium mining site in Thuringia, Germany. The strains from a metal-rich environment were not only highly salt tolerant (up to 20% NaCl), but at the same time could sustain elevated Cs and Sr (both up to 100 mM) concentrations as well as other (heavy) metals present in the environment. During growth experiments when screening for differential cell morphology, the occurrence of guttation droplets was observed, specifically when elevated Sr concentrations of 25 mM were present in the media. To analyze the potential of metal tolerance being promoted by these excretions, proteomics and metabolomics of guttation droplets were performed. Indeed, proteins involved in up-regulated metabolic activities as well as in stress responses were identified. The metabolome verified the presence of amino sugars, glucose homeostasis-regulating substances, abscisic acid and bioactive alkaloids, flavones and quinones.

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